Fig. 3

Effect of IL-1β inhibition on chemokine expression following photo-oxidative damage (PD). a In animals that were intravitreally injected with Il-1β-specific siRNA, there was significantly lower expression of Ccl2 and Cxcl1 compared to control siRNA after photo-oxidative damage (P < 0.05). In the group injected with IL-1β neutralising antibody however, the expression of Ccl2, Cxcl1 and Cxcl10 were all significantly down-regulated in comparison to the isotype control group (P < 0.05). b-k In situ hybridisation was used to examine the localisation of Ccl2 and Cxcl1 mRNA transcripts following IL-1β inhibition and photo-oxidative damage, as shown in representative images. Staining for Ccl2 mRNA (purple/red) was decreased in IL-1β antibody-injected retinas compared to control IgG retinas (b-c; arrows), and was co-localised to vimentin–immunoreactive Müller cells (green) (D-E; arrows). Staining for Cxcl1 mRNA was observed within INL (f-g) and RPE layers (h-i), which was decreased in the IL-1β neutralising antibody group. The INL staining correlated with Müller cell processes that were immunolabelled with vimentin (j-k). INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segments. N = 5 per group. Asterisks denote a significant change, where P < 0.05