Table 1 Antibodies used for western blotting (WB), immunocytochemistry (ICC), or proximity ligation assay (PLA)
Antibody/Clone | Target | Source | Final Concentration/ Dilution | Method |
|---|---|---|---|---|
AHB0042 (clone: Tau5) | Tau | Thermo Fisher Scientific | 1 μg/ml | WB, ICC, PLA |
MAB10417 (clone: EP2456Y) | Tau | EMD Millipore | 0.1 μg/ml | WB |
MAB302 (clone: GS-6) | Glutamine Synthetase | EMD Millipore | 1:1000 | ICC |
MAB3420 (clone: Tau1-PC1C6) | Tau | EMD Millipore | 1 μg/ml | WB, ICC, PLA |
MAB377 (clone: A60) | NeuN | EMD Millipore | 1:1000 | ICC |
sc-16 (clone: Fyn3) | Fyn | Santa Cruz Biotechnology | 1 μg/ml 4 μg/ml | WB, PLA |
T5076-200UL (clone: SDL.3D10) | βIII Tubulin | Sigma-Aldrich | 0.1 μg/ml | WB |
Secondary antibodies conjugated with Alexa Fluor | Respective species and isotype | Thermo Fisher Scientific | 2 μg/ml | ICC |
Secondary antibodies conjugated with IRDye | Respective species and isotype | LI-COR | 0.1 μg/ml | WB |