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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Dioxins and related environmental contaminants increase TDP-43 levels

Fig. 3

Benzo(a)pyrene treatment increases endogenous TDP-43 levels in M17 cells and in the murine brain. a Immunoblot of total lysates of M17 neuroblastoma cells, differentiated for 7 days in 10 μM RA, then treated for 7 days with vehicle, the AHR-activating toxin Benzo(a)pyrene (B(a)P; 10 μM), or with B(a)P and CB7993113, the AHR antagonist (10 μM). b Immunoblot of cortical tissue from mice exposed by intraperitoneal (i.p) injection to the AHR agonist Benzo(a)pyrene. Densitometry of monomeric TDP-43 bands shown in the immunoblots were quantified in c for the M17 cell samples (N = 3; mean ± SEM, ANOVA w/ Tukey’s; ** P < 0.01) and in d (for the murine cortical tissue (N = 4; mean ± SEM, ANOVA w/Tukey’s; ** P < 0.01, * P < 0.05). The environmental toxin, B(a)P, increases levels of endogenous TDP-43 protein in both cultured human cells and in the brains of mice exposure by intraperitoneal injection. This increase is substantially reversed in each model system by co-treatment with the AHR antagonist CB7993113

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