Table 1 Experimental parameters for H129-G4 tracing
From: Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129
Animal | Nucleus | Coordinates (mm) | Dose (pfu) | Vol. (μl) | Perfusion time | Animalnumber | ||
|---|---|---|---|---|---|---|---|---|
ML | AP | DV | (dpi) | |||||
C57BL/6 | M1 | -1.70 | +1.54 | −1.75 | 1.0 × 106 | 0.2 | 4 | 3 |
C57BL/6 | retina | NA | NA | NA | 5.0 × 106 | 1.0 | 4 | 4 |
C57BL/6 | LGN | +2.13 | −2.30 | −2.75 | 5.0 × 104 / 5.0 × 105 | 0.1 | 3 and 5 | 4 |
C57BL/6 | V1 | +2.30 | −3.52 | −1.15 | 5.0 × 104 / 5.0 × 105 | 0.1 | 4 and 8 | 4 |
C57BL/6 | VPM | +1.40 | −1.82 | −3.62 | 1.0 × 106 | 0.2 | 2–5 | 8 |
Tree shrew | M1 | −2.60 | +1.85 | −2.10 | 2.0 × 106 | 0.3 | 6 | 5 |