Table 2 Experimental parameters for monosynaptic tracing
From: Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129
Mouse | Nucleus | Coordinate (mm) | Helper virus (1 × 1012 v.g./ml) | H129-ΔTK-tdT | Examination time (Day) | Animalamount | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ML | AP | DV | Type | Vol. (μl) | Injection (Day) | Dose (pfu) | Vol. (μl) | Injection (Day) | Starterneuron | Postsynapticneuron | |||
C57BL/6 | MOB | -0.48 | +4.28 | -2.30 | AAV-TK-GFP | 0.45 | Day 1 | 1.00 × 105 | 0.40 | Day 22 | 25 | 29 | 4 |
C57BL/6 | LGN | +2.13 | −2.30 | -2.75 | AAV-TK-GFP | 0.20 | Day 1 | 1.00 × 105 | 0.20 | Day 22 | 25 | 32 | 4 |
C57BL/6 | VPM | -1.42 | −1.82 | -3.55 | AAV-TK-GFP | 0.20 | Day 1 | 1.00 × 105 | 0.20 | Day 22 | 25 | 32 | 5 |
PV-Cre | nRT | +1.50 | −0.82 | -3.75 | AAV-DIO-TK-GFP | 0.35 | Day 1 | 1.25 × 105 | 0.25 | Day 22 | 25 | 32 | 5 |
DAT-Cre | VTA | −0.40 | −3.28 | -4.30 | AAV-DIO-TK-GFP | 0.35 | Day 1 | 1.25 × 105 | 0.25 | Day 22 | 25 | 32 | 5 |