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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Inhibition of O-GlcNAcase leads to elevation of O-GlcNAc tau and reduction of tauopathy and cerebrospinal fluid tau in rTg4510 mice

Fig. 2

Determination of OGA enzyme and O-protein levels in OGA iKD mice. a Binding of [3H]Thiamet G in brain homogenates prepared from WT (n = 5) and OGA iKD (n = 4) mice was determined as described in Methods. Individual saturation binding curves were performed using total brain homogenates prepared from each animal and the data shown are the mean ± standard deviation of values for each group of animals. The KD of [3H]Thiamet G binding to OGA was similar in WT and OGA iKD mouse brain (KD = 1.7 nM and 1.9 nM, respectively), while the Bmax values were reduced by 80% in the OGA iKD mouse relative to the WT mouse (433 fmol/mg protein and 78 fmol/mg protein, respectively). b Total O-protein in brain homogenates from WT (n = 5) and OGA iKD mice (n = 5) was measured by a quantitative sandwich immunoassay as described in Methods. Brain O-protein levels were 1.4-fold higher in OGA iKD mice relative to the WT mice. The data shown are the mean ± SEM. Each sample was assayed in duplicate. *p = 0.048 (t-test)

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