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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: Inhibition of O-GlcNAcase leads to elevation of O-GlcNAc tau and reduction of tauopathy and cerebrospinal fluid tau in rTg4510 mice

Fig. 7

Validation of the “click” chemistry method for detection of O-tau. a Flow chart describing the procedure for O-tau detection (see Methods for further details). b Detection of a 55 kDa TAMRA-labeled protein in rTg4510 mouse brain homogenate (upper panel) that is recognized by the HT7 anti-tau antibody (lower panel). Note that this putative human O-tau band migrates at the known molecular weight of soluble human tau (55 kDa) and was seen only in brain samples from rTg4510 mice that overexpress human tau (lanes labeled TG), but not in brain samples from wild type C57BL6 mice (lanes labeled WT). c The 55 kDa TAMRA labeled band was insensitive to PNGase F treatment (PNG), required Gal-T1 treatment and was abolished by β-elimination (β-EL), thus further validating this band as O-tau

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