Fig. 5

Modest levels of nigral DA neuronal loss in line M20 mice injected in IC with αSyn fibrils. Line M20 mice were injected in the IC (Cohorts 1 and 2) or CPu (Cohort 3) with WT αSyn fibrils. Representative TH staining depicting SN-resident DA neurons in the dorsal (left panel) and ventral aspects (right panel) are shown. Mice that were injected with either human αSyn fibrils (Cohort 1) or mouse αSyn fibrils (Cohort 2) showed modest levels of DA degeneration as determined by counting TH-immunostained neurons in the SN (~20–25% compared to PBS injected control mice). The thinning of the nigral TH neuron layer is indicated by arrows. Cohort 3 mice injected with human αSyn fibrils did not display any nigral degeneration. PBS injected Line M20 mice were considered as control group for all cohorts. Scale Bar, 100 μm; n = 3–5/cohort. One way Anova, **p < 0.01, *p < 0.5