Fig. 7

Increased astrogliosis in line M20 mice injected with αSyn fibrils. Representative images of astrocytosis (GFAP immunostaining, a-b) and microgliosis (Iba-1 immunostaining, c-d) in the striatum region of line M20 (a, c) or NTG (b, d) mice injected with αSyn fibrils. Astrocytic and microglial activation was significantly upregulated in line M20 mice injected in the CPu with human αSyn fibrils (Cohort 3). Astrogliosis was variable in Cohort 1 of line M20 mice whereas in Cohort 2 of M20 mice, injection of mouse αSyn fibrils in the IC resulted in an insignificant trend towards increased GFAP and Iba-1 reactive astroglia. In all cohorts of NTG mice, the extent of astrogliosis was more variable (Cohorts 4, 5 and 6) and in Cohort 6, mice showed significantly lower microgliosis compared to control group. In all cases, genotype-matched PBS injected mice served as controls. Different cohorts are identified by numbers in parenthesis as described in Figs. 1 and 3. Scale Bar, 100 μm; n = 3–5/cohort; 1 way Anova, ***p < 0.005, **p < 0.01