Fig. 3
From: A C9ORF72 BAC mouse model recapitulates key epigenetic perturbations of ALS/FTD
Hexanucleotide (GGGGCC) repeat methylation increases with age in C9-BAC mice. An illustration of the HRE methylation assessment method. For each sample, two repeat-primed PCR reactions are performed, one using DNA subjected to HpaII digest and one using uncut control (UC). DNA methylation percentage is calculated based on the area under the curve values following capillary fragment analysis of HpaII digested samples normalized to the uncut control sample (a). HRE methylation levels were assessed at 0 (n = 5), 2 (n = 4) and 7 (n = 5) weeks of age, significance is indicated by *p < 0.05 (b). Representative electropherograms from 0 to 7 week old C9-BAC mouse brain cortex DNA treated with HpaII as well as uncut control (c)