Fig. 6

Absent correlation of age and binding properties of anti-α-synuclein NAbs in plasma from negative controls. Percentage of maximal plasma NAbs binding to immobilized α-synuclein monomer (5 μg/mL) in younger controls (<50 years old, n = 26) and in elderly controls (>50 years old, n = 15) determined by competitive ELISA assay with a, b 50 nM or c, d 2 nM of free α-synuclein, where 10,000 nM defines the non-specific binding and no free α-synuclein monomer gives the maximal binding. Horizontal bars represent the mean values ± SEM. Plasma samples were diluted tested in two different dilutions: a, c 1:100 and b, d 1:200