Fig. 3
From: Microglia limit the expansion of β-amyloid plaques in a mouse model of Alzheimer’s disease
Microglia depletion induces increase in plaque size. a. Blue curve in the bottom panel indicates the identified plaque border for the example in the upper panel. The border of amyloid plaque was determined such that the fluorescence intensity of amyloid plaques was above 3 times of s.d. b. Distribution of plaque size on Day 13 in the cortex of APP/PS1 mice (green bars, n = 129 plaques from 5 mice) and APP/PS1/CX 3 CR1-iDTR mice (red bars, n = 75 plaques from 4 mice) (P = 0.23, Mann-Whitney test). c. Three examples of plaques imaged over 1–2 weeks in APP/PS1 mice. d. Three examples of plaques imaged over 1–2 week in APP/PS1/CX 3 CR1-iDTR mice. e. Change of plaque size in the first week after DT administration. No significant change in plaque size was detected within one week in APP/PS1 mice (n = 129 plaques from 5 mice, P = 0.16, Wilcoxon matched-pairs signed rank test). Microglia depletion induced a significant increase in plaque size in APP/PS1/CX 3 CR1-iDTR mice (n = 75 plaques from 4 mice, ***P < 0.001, Wilcoxon matched-pairs signed rank test). The distribution of plaque size at D20 relative to D13 is significantly different between these two groups (right panel, ** P < 0.01, Mann-Whitney test). f. Change of plaque size in the second week after DT administration. No significant change in plaque size was detected in APP/PS1 mice (n = 92 plaques from 5 mice, P = 0.52, Wilcoxon matched-pairs signed rank test). Aβ plaques in APP/PS1/CX 3 CR1-iDTR mouse cortex didn’t continue to grow from D20 to D27 (n = 40 plaques from 4 mice, P = 0.32, Wilcoxon matched-pairs signed rank test). The distribution of plaque size at D27 relative to D20 is not significantly different between these two groups (right panel, P = 0.52, Mann-Whitney test). Scale bar in a, c and d, 20 μm