Fig. 3

Impaired PTP at hippocampal MF synapses in PS cDKO mice. a & b Representative data showing the time course of PTP and the effect of CGP37157 (20 μM) on PTP of EPSCs obtained by whole-cell patch recording at the hippocampal MF synapses in control and PS cDKO mice. DCG IV (2 μM) was applied at the end of each experiment to confirm that MF synapses were recorded. The insets represent EPSC traces recorded the baseline (1) and immediately after HFS induction (2 & 3). Scale bar: 20 ms, 200 pA. c Summary bar graphs of the mean magnitude of PTP in the course of whole-cell patch recording in control and PS cDKO mice. PTP is impaired at MF synapses in PS cDKO mice. d Depicted EPSC trace for the estimation of latency, 10–90% rise time and decay time constant (τ). e Histogram of latency between stimulus onset and EPSC response was analyzed during 1 min baseline recordings of EPSC (n = 72 in 6 PTP recordings) in both control and PS cDKO mice. Latencies ranged from 1.6 to 2.5 ms with means of 1.93 ± 0.03 and 2.01 ± 0.03 ms in the control and PS cDKO mice, respectively. f The 10–90% rise time for control EPSCs (2.27 ± 0.06 ms range from 1.19 to 3.13 ms, n = 72) and PS cDKO EPSCs (2.29 ± 0.05 msec range from 1.38 to 3.14 ms, n = 72) is shown. g Relationship of 10–90% decay time constant (τ) and Δ amplitude of EPSCs. The distribution was well fitted with linear function (Black dots for control: y = 0.019 x + 6.288, n = 72; red dots for PS cDKO: y = 0.016 x + 7.005, n = 72). All data represent means ± SEM (** p < 0.01, NS: not significant; Student’s t-test). The values in parentheses indicate the number of hippocampal neurons (left) and the number of mice (right) used in each experiment