Table 1 Previously reported LRRK2 binding partners from the Overexpressed LRRK2 MS/MS data set were confirmed our “Overexpressed LRRK2 in-gel” data set, proving the quality and validity of the experiment
PROT_HIT | ACCESSION | PROTEIN NAME | REF | METHOD, MODEL |
|---|---|---|---|---|
27 | TBB4A_HUMAN | Tubulin beta-4A chain | [103] | MS and Co-IP of overexpressed LRRK2-WT in HEK293T |
35 | JIP4_HUMAN | C-Jun-amino-terminal kinase-interacting protein 4 | [40] | Overexpression with LRRK2-WT in HEK293T |
56 | RIF1_HUMAN | Telomere-associated protein RIF1 | [104] | MS and Co-IP of overexpressed G2019S in HEK293T |
180 | MYBB_HUMAN | Myb-related protein B | [104] | Its interactor RBBP4-6-7 binds to LRRK2. MS and Co-IP of overexpressed G2019S in HEK293T |
184 | H90B2_HUMAN | Putative heat shock protein HSP 90-beta 2 | [105] | Co-IP of endogenous LRRK2 from forebrain and overexpressed in HEK293T |
194 | JIP3_HUMAN | C-Jun-amino-terminal kinase-interacting protein 3 | [40] | Overexpression with LRRK2-WT in HEK293T |
215 | CENPF_HUMAN | Centromere protein F | [104] | MS and Co-IP of overexpressed G2019S in HEK293T |