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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: TRPA1 channels promote astrocytic Ca2+ hyperactivity and synaptic dysfunction mediated by oligomeric forms of amyloid-β peptide

Fig. 7

Aβo increases the frequency of spontaneous excitatory post-synaptic currents (sEPSCs) from CA1 pyramidal neurons in a TRPA1 channels dependent manner. a Representative traces of voltage-clamp recordings from CA1 pyramidal cells held at −65 mV in physiological condition (grey) and under Aβo 100 nM application (orange). Examples of single EPSC with higher time resolution are shown in the corresponding insets. b Time course of the frequency of sEPSCs in physiological condition (light grey; n = 5), under application of 100 nM Aβo (orange; n = 7), 40 μM HC 030031 (dark grey; n = 5) or co-application of 100 nM Aβo + 40 μM HC 030031 (cyan; n = 4). c Histogram showing the sEPSCs frequency at a time matching the astrocyte calcium activity measurements (i.e. 5 to 10 min after drugs application). Results are compared with the physiological condition with *, p < 0.05; **, p < 0.01 and ***, p < 0.001 or the Aβo condition with #, p < 0.05; ##, p < 0.01 and ###, p < 0.001. d Representative traces of voltage-clamp recordings from CA1 pyramidal cells held at −65 mV in APP/PS1–21 mice (orange) and their WT littermates (grey). Examples of single EPSC with higher time resolution are shown in the corresponding insets. e Time course of the normalized frequency of sEPSCs in WT (dark grey; n = 7) and APP/PS1–21 mice (cyan; n = 8) under application of 40 μM HC 030031. f Histogram showing the sEPSCs frequency in basal condition in WT (light grey; n = 7), APP/PS1–21 mice (orange; n = 8) and 5 to 10 min after HC 030031 application (dark grey and cyan respectively). Results are compared to the WT basal activity with *, p < 0.05; **, p < 0.01 and ***, p < 0.001 or the APP/PS1–21 basal activity with #, p < 0.05; ##, p < 0.01 and ###, p < 0.001

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