Fig. 3

LPS, but not MPLA induces re-distribution of α-syn from soluble monomeric to insoluble and oligomeric species. a Brains of PLP-α-syn mice were lysed and three protein fractions were prepared – TX soluble fraction, SDS soluble fraction, and Urea fraction. Membranes were probed for four different α-syn antibodies (pS129, 4B12, Syn1, and C20). Relative density (RD) for monomeric α-syn (15 kDa) and oligomeric high molecular weight α-syn (HMW, over 30 kDa) was normalized to the intensity of the β-actin band (40 kDa). b No significant effects of the treatments on α-syn distribution were identified in the TX soluble fraction. c LPS treatment induced significant reduction of the SDS soluble monomeric α-syn and a tendency towards increase of the HMW SDS soluble α-syn species. MPLA treatment had no significant effect on the distribution of α-syn species in the SDS fraction. d In the urea fraction a significant increase of insoluble α-syn species was identified after LPS treatment, but not after MPLA systemic treatment of PLP-α-syn mice. Data are means ± SEM, n = 4 per group. * p < 0.05, ** p < 0.01 compared to vehicle treated mice