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Table 1 Summary of identified peptides from Cat L proteolytic processing of PGRN

From: The lysosomal protein cathepsin L is a progranulin protease

  Peptide Sequence location QS SEQUEST XCorr Mascot Ion Score Δ mass (ppm)
(i) DKWPTT (Linker P – G)
 CL-1 DKWPTT 47–52 2 1.46 20 −0.39
(ii) ITPTGTHPLAKKLPAQR (Linker F – B)
 CL-2 ITPTGTHPLA 179–188 2 1.33 23 0.65
 CL-3 KKLPAQR 189–195 2 1.78 31 −0.43
 CL-4 KLPAQR 190–195 2 1.49 31 −0.29
(iii) EQGPHQVPWM (Linker A – C)
 CL-5 EQGPHQVPWM(ox) 336–345 2 1.54 23 0.23
(iv) LPDPQALK (Linker A – C)
 CL-6 LPDPQALK 354–361 2 1.73 36 0.20
 CL-7 LPDPQAL 354–360 2 1.86 30 −0.64
 CL-8 LPDPQA 354–359 1 1.57 30 −0.26
(v) AQPATF (Linker D – E)
 CL-9 AQPATF 502–507 1 1.47 22 0.53
(vi) EAPRWDAPLR (Linker C-terminus end)
 CL-10 EAPRWDAPLR 576–585 3 1.83 25 0.13
  1. QS: Charge state, (ox) methionine oxidation, Δ mass (ppm), difference between experimental and theoretical masses in parts-per-million. Annotated MS/MS spectra of the identified granulin peptides generated by Cat L activity were included in Additional file 1: Figure S2, numbered from CL-1 to CL-10