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Table 1 Summary of identified peptides from Cat L proteolytic processing of PGRN

From: The lysosomal protein cathepsin L is a progranulin protease

 

Peptide

Sequence location

QS

SEQUEST XCorr

Mascot Ion Score

Δ mass (ppm)

(i) DKWPTT (Linker P – G)

 CL-1

DKWPTT

47–52

2

1.46

20

−0.39

(ii) ITPTGTHPLAKKLPAQR (Linker F – B)

 CL-2

ITPTGTHPLA

179–188

2

1.33

23

0.65

 CL-3

KKLPAQR

189–195

2

1.78

31

−0.43

 CL-4

KLPAQR

190–195

2

1.49

31

−0.29

(iii) EQGPHQVPWM (Linker A – C)

 CL-5

EQGPHQVPWM(ox)

336–345

2

1.54

23

0.23

(iv) LPDPQALK (Linker A – C)

 CL-6

LPDPQALK

354–361

2

1.73

36

0.20

 CL-7

LPDPQAL

354–360

2

1.86

30

−0.64

 CL-8

LPDPQA

354–359

1

1.57

30

−0.26

(v) AQPATF (Linker D – E)

 CL-9

AQPATF

502–507

1

1.47

22

0.53

(vi) EAPRWDAPLR (Linker C-terminus end)

 CL-10

EAPRWDAPLR

576–585

3

1.83

25

0.13

  1. QS: Charge state, (ox) methionine oxidation, Δ mass (ppm), difference between experimental and theoretical masses in parts-per-million. Annotated MS/MS spectra of the identified granulin peptides generated by Cat L activity were included in Additional file 1: Figure S2, numbered from CL-1 to CL-10