Skip to main content

Table 2 Summary of identified peptides from elastase proteolytic processing of PGRN

From: The lysosomal protein cathepsin L is a progranulin protease

 

Peptide

Sequence location

QS

SEQUEST XCorr

Mascot Ion Score

Δ mass (ppm)

(i) LDKWPTLSRHLG (Linker P – G)

 EL-1

LDKWPT

45–50

2

1.33

29

−0.58

 EL-2

TLSRHLG

51–57

2

1.43

24

−0.62

(ii) KLPAHTV (Linker B – A)

 EL-3

KLPAHTV

273–279

2

1.42

29

−0.33

(iii) PWMEKAPAHLSLPDPQAL (Linker A – C)

 EL-4

PWMEKAPA

343–350

2

1.73

23

0.55

 EL-5

PWM(ox)EKA

343–348

2

1.37

28

−1.70

 EL-6

AHLSLPDPQ

350–358

2

1.93

23

−0.57

 EL-7

HLSLPDPQAL

351–360

2

3.22

70

1.3

 EL-8

HLSLPDPQA

351–359

2

1.81

36

1.2

 EL-9

HLSLPDPQ

351–358

2

1.97

38

0.79

 EL-10

HLSLPDP

351–357

2

1.61

40

−1.3

 EL-11

HLSLP

351–355

1

1.34

23

1.0

 EL-12

LSLPDPQ

352–358

1

1.75

35

0.23

(iv) VAGLEKMPA (Linker (Linker C – D)

 EL-13

VAGLEKM(ox)PA

423–431

2

1.41

32

0.79

(v) TFLARSPHVGV (Linker D – E)

 EL-14

TFLARSPHV

506–514

2

1.89

34

0.53

 EL-15

RSPHVGV

510–516

2

1.82

33

−0.24

(vi) RWDAPLRDPAL (Linker C-terminus end)

 EL-16

RWDAPLRDPAL

579–589

2

2.55

21

−0.19

 EL-17

RWDAPLRDPA

579–588

3

2.94

50

2.0

 EL-18

RWDAPLRDP

579–587

2

2.38

24

0.52

 EL-19

RWDAPLR

579–585

2

1.98

26

−1.1

  1. QS: Charge state, M(ox) methionine oxidation, Δ mass (ppm), difference between experimental and theoretical masses in parts-per-million. Annotated MS/MS spectra of the identified granulin peptides generated by elastase activity were included in Additional file 1: Figure S2, numbered from EL-1 to EL-19