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Table 2 Summary of identified peptides from elastase proteolytic processing of PGRN

From: The lysosomal protein cathepsin L is a progranulin protease

  Peptide Sequence location QS SEQUEST XCorr Mascot Ion Score Δ mass (ppm)
(i) LDKWPTLSRHLG (Linker P – G)
 EL-1 LDKWPT 45–50 2 1.33 29 −0.58
 EL-2 TLSRHLG 51–57 2 1.43 24 −0.62
(ii) KLPAHTV (Linker B – A)
 EL-3 KLPAHTV 273–279 2 1.42 29 −0.33
(iii) PWMEKAPAHLSLPDPQAL (Linker A – C)
 EL-4 PWMEKAPA 343–350 2 1.73 23 0.55
 EL-5 PWM(ox)EKA 343–348 2 1.37 28 −1.70
 EL-6 AHLSLPDPQ 350–358 2 1.93 23 −0.57
 EL-7 HLSLPDPQAL 351–360 2 3.22 70 1.3
 EL-8 HLSLPDPQA 351–359 2 1.81 36 1.2
 EL-9 HLSLPDPQ 351–358 2 1.97 38 0.79
 EL-10 HLSLPDP 351–357 2 1.61 40 −1.3
 EL-11 HLSLP 351–355 1 1.34 23 1.0
 EL-12 LSLPDPQ 352–358 1 1.75 35 0.23
(iv) VAGLEKMPA (Linker (Linker C – D)
 EL-13 VAGLEKM(ox)PA 423–431 2 1.41 32 0.79
(v) TFLARSPHVGV (Linker D – E)
 EL-14 TFLARSPHV 506–514 2 1.89 34 0.53
 EL-15 RSPHVGV 510–516 2 1.82 33 −0.24
(vi) RWDAPLRDPAL (Linker C-terminus end)
 EL-16 RWDAPLRDPAL 579–589 2 2.55 21 −0.19
 EL-17 RWDAPLRDPA 579–588 3 2.94 50 2.0
 EL-18 RWDAPLRDP 579–587 2 2.38 24 0.52
 EL-19 RWDAPLR 579–585 2 1.98 26 −1.1
  1. QS: Charge state, M(ox) methionine oxidation, Δ mass (ppm), difference between experimental and theoretical masses in parts-per-million. Annotated MS/MS spectra of the identified granulin peptides generated by elastase activity were included in Additional file 1: Figure S2, numbered from EL-1 to EL-19