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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Tau accumulation in the retina promotes early neuronal dysfunction and precedes brain pathology in a mouse model of Alzheimer’s disease

Fig. 2

Retinal tau undergoes epitope-specific and age-dependent phosphorylation changes in AD. a-c Western blot analyses of retinal homogenates probed with the phospho-tau specific antibodies AT8, PS199, and PHF1 revealed alterations in tau phosphorylation in 3xTg relative to wild-type (WT) mice. d-f Densitometry analysis of phospho-tau relative to total tau revealed increased tau phosphorylation on S202/T205 (AT8) and reduced phospho-S199 (PS199) in 3xTg retinas relative to controls, while no change was detected on S396/S404 (PHF1) (3xTg: n = 5, WT: n = 4, Student’s t-test, * = p < 0.05, ** = p < 0.01). g-i Western blot analysis of tau phosphorylation in 6-month-old 3xTg retinas showed alterations on epitopes AT8 and PS199, but not PHF1, relative to controls. j-l Quantitative analysis confirmed decreased tau phosphorylation on S202/T205, increased phosphorylation on S199, and no change on PHF1 (3xTg: n = 5, WT: n = 4, Student’s t-test, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, n.s.: not significant p > 0.05). Vertical lines represent non-consecutive samples from the same gel

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