Fig. 4

Tau accumulates in the somatodendritic compartment of RGCs. a-d Retinal immunohistochemistry using antibodies against total tau (K9JA) revealed marked tau upregulation in 3- and 6-month-old 3xTg retinas relative to age-matched wild-type (WT) controls. e-g Co-staining of tau with TUJ1, an RGC-specific marker, demonstrated tau accumulation in 3xTg RGC dendrites and somata (arrowheads) (n = 5/group). h-s Whole-mount retinal preparations from 3- and 6-month-old mice show co-localization of tau and RBPMS, a selective marker of RGC soma, demonstrating age-dependent accumulation of tau in RGC soma (n = 5/group). Scale bars: A–D = 50 μm, E-G = 25 μm, H-S = 50 μm. ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer