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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Absence of CX3CR1 impairs the internalization of Tau by microglia

Fig. 1

The absence of CX3CR1 leads to a decrease in extracellular Tau internalization by microglia in vitro. Representative immunofluorescence images of primary cultures of microglia derived from WT mice (a) and their high-power magnification (b), and from KO animals (c) and their magnification image (d). Cells were incubated with 1 μM Tau-Cy5. Immunofluorescence images for Iba1 (white) and Cy5 (red) are shown separately. (e) Quantification of Cy5+ area in individual microglial cells treated with either Tau-Cy5, or BSA-Cy5 (f) for 0, 5, 30 and 60 min. g-l Representative images of primary microglia cultures from WT mice treated with PBS-Cy5 (g) or Tau-Cy5 (h) and from KO animals treated with PBS-Cy5 (i) or Tau-Cy5 (j) showing CD68 (white) and Iba1 (green) staining. k Quantification of CD68+ area in individual WT and KO microglia after treatment with PBS or Tau at 0 and 30 min. Bars show means ± S.E. p ≤ 0.001. White scale bar: 100 μm, purple scale bar: 50 μm

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