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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Absence of CX3CR1 impairs the internalization of Tau by microglia

Fig. 3

Tau binds to CX3CR1. Synthetic CX3CR1 was coupled to CNBr-activated Sepharose4B. Tau detected with anti-Tau5 (a), the positive control CX3CL1 (b), the negative control c-terminal Tau detected with anti-Tau46 (c), and Tau phosphorylated by GSK-3β detected with anti-pTau396 (d) were loaded on the column, as described in the Methods section. Each protein sample was used at a concentration of 1 mg/ml. Exclusion volume (excl. Vol.), aliquots corresponding to the column washed with buffer A, bound proteins eluted by addition of 0.5 M NaCl (marked with an arrow), and collected fractions (Fract. N°) are shown. Note that the profiles indicate that Tau, CX3CL1 and phospho-Tau were bound to the column, whereas the C-terminal fragment of Tau was not

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