Skip to main content
Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Role of the sigma-1 receptor chaperone in rod and cone photoreceptor degenerations in a mouse model of retinitis pigmentosa

Fig. 2

Up-regulation of RIP1 and RIP3 proteins and their interaction (proximity ligation) in rd10 retinas due to S1R knockout. Animals were euthanized at indicated time points, and retinal sections or homogenates were prepared for RIP1/RIP3 proximity ligation assay and Western blotting, respectively. a. Proximity ligation. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. Scale bar: 20 μm. Quantification: mean ± SE, n = 3–4 mice; *P < 0.05. b. Quantification of Western blots. Shown is one of four blots each representing an independent experiment. Each lane represents one mouse, from which two retinas were homogenized together and subjected to Western blotting. Normalized (to beta-actin) values of the same condition from four experiments (blots) were averaged to calculate mean ± SE; n = 4 mice; **P < 0.01, ***P < 0.001

Back to article page