Fig. 5

Changes of LC3-II and CHOP protein levels during PR degeneration in rd10 retinas with or without S1R. a and c. Western blots detecting LC3-I (uncleaved), LC3-II (cleaved and lipidated, marker of autophagosomes), and CHOP in retinal homogenates collected at indicated time points. The postnatal day-19 experiment (see a) was performed about 6 months after that in c, triplicate samples (each from two retinas in one mouse) were used for the same blot. b and d. Quantification of Western blots shown in a and c, respectively. For the method of quantification refer to Fig. 2b; mean ± SE; n = 4 mice; *P < 0.05, **P < 0.01, ***P < 0.001. e. Immunostaining of LC3 on retinal sections collected at indicated time points