Fig. 1
From: Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A
Aβ increases MVB diameter in primary neurons. a Electron microscopy reveals that APP/PS1 AD transgenic compared to wt mouse primary neurons have significantly larger MVBs at 12 DIV. Scale bar 500 nm. b Quantification of A shows an increase of 52% in MVB diameter in Tg compared to wt neurons, n > 94 MVBs per group; *** p < 0.001. c Exogenously added monomeric Aβ1-40 or Aβ1-42 to wt primary neurons at 12 DIV leads to increased MVB diameter at 48 h on EM images. MVBs are marked with an asterisk. Scale bar 500 nm. d Quantification of C, n > 45 MVBs per group; *** p < 0.001. e Shorter time-points of exogenously added monomeric Aβ1-42 already leads to increased size of LAMP1-positive vesicles 3 h after treatment in wt mouse neurons at 12 DIV; 3D-rendering with Imaris from confocal z-stack. High magnification image of LAMP1 labelling from a single focal plane to the right. Scale bar 10 μm. f Quantification of E shows significant increase in diameter of LAMP1-positive vesicles after addition of Aβ. The diameters of the five largest LAMP1-positive vesicles per pyramidal neuron were measured in the z-stacks; n > 10 neurons per treatment; **** p < 0.0001