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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A

Fig. 7

Induction of autophagy partially rescues dnVPS4A induced intracellular accumulation of Aβ. a Expression of dnVPS4A increases levels of LC3β-II, an indicator of autophagy, by 35% (n = 3, *p < 0.05). Representative blot (above) and quantification (below); values are normalized against actin and expressed as percentage of ctrGFP. b dnVPS4A increases levels of p62, an indicator of autophagy, by 52% (n = 3, **p < 0.01). Representative blot (above) and quantification (below); values are normalized against actin and expressed as percentage of ctrGFP. c Chemically induced autophagy by Rapamycin partially rescues the increase in intracellular Aβ from dnVPS4A. 6 h after transfection Swe N2a cells were treated with 1 μM Rapamycin and harvested 24 h post-transfection. Representative blot (above) and quantification (below) demonstrating 35% reduction in intracellular Aβ by Rapamycin on dnVPS4 transfected cells. Values are normalized against actin and expressed as percentage of dnVPS4 (n = 6, **p < 0.01). d Induced autophagy by torin 1 or by starvation significantly reduces intracellular Aβ to levels in dnVPS4 transfected cells. Values are normalized against actin and expressed as percentage of control transfected cells (n = 3, *p < 0.05, **p < 0.01)

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