Fig. 2

A significant reduction of amyloid-beta plaques in the Gad67 haploinsufficiency 5xFAD mouse brains. a Images of the sagittal section of whole brain showing decreased Aβ42 deposits (white) in AD+GG- (top) and AD+GG+ (bottom) mice of 10–11 months age. b Representative confocal images display the level of Aβ42 deposits (red) in the four genotypes (AD-GG-, AD-GG+, AD+GG-, AD+GG+) generated by crossing of GAD67-GFP knock-in mice with 5xFAD mice. GFP cells showed in green. Scale bar = 30 μm. c Quantification analysis showing a remarkable reduction of Aβ42 (left panel and right panel, Aβ42 intensity and Aβ42 covered area percentage, respectively) in the bigenic (AD+GG+) mice brain than 5xFAD mice of similar age. N = 6 mice of 10–11 months age in each genotype groups (AD-GG-, AD-GG+, AD+GG-, AD+GG+). d Elisa data also showing the Aβ40 and Aβ42 were significantly reduced in 5xFAD mice by Gad67 haploinsufficiency (unpaired Student’s t test). Data are presented as mean ± s.e.m., * p < 0.05; ** p < 0.01; *** p < 0.001; one-way ANOVA with the Tukey’s post-hoc test when comparing multiple groups