Fig. 7

Rescue of olfactory deficit in 5xFAD mice with Gad67 haploinsufficiency. Olfactory behavior test is applied to examine the extent of olfactory sensory impairment in the four genotypes of 6–8 months age (AD-GG-, AD-GG+, AD+GG-, AD+GG+). a Statistical graph showing the bigenic (AD+GG+) mice displayed a reduction of investigation time in each odor presentation trials than 5xFAD mice (red line). b Quantification data showing the AD+GG+ mice displayed a reduction of investigation time (normalized) (blue line) than 5xFAD mice (red line). Notably, AD+GG+ mice displayed almost recovery of the impairment olfactory sensory function (which is a common pathological symptom in 5XFAD), according to the result that the statistic values are very close to that of AD-negative mice (black line: AD-GG-; grey line: AD-GG+). c AD+GG+ displayed a recovered cross-habituation index (mean ± s.e.m., normalized). For (A)-(C), n = 12 AD-GG-, 20 AD-GG+, 14 AD+GG-, 23 AD+GG+ mice of 6–8 months age. * p < 0.05; ** p < 0.01; *** p < 0.001; two-way ANOVA followed by LSD post hoc test for comparison among multiple groups for multiple conditions