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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Together JUN and DDIT3 (CHOP) control retinal ganglion cell death after axonal injury

Fig. 1

Jun and Ddit3 deficiency does not alter retinal morphology. a Semi-thin retinal cross sections were taken to evaluate the gross structure of the retina in WT, Jun, Ddit3, and Jun/Ddit3 deficient animals. Jun, Ddit3, and combined Jun/Ddit3 deficiency did not appear to alter gross retinal structure. b TUJ1+ cells (a marker of RGCs) were counted in retinal flat mounts in WT, Jun, Ddit3, Jun/Ddit3 deficient animals. No difference was observed across genotypes (P > 0.05 for each comparison; n = 8 per group). c/d JUN accumulation was readily detected in the somas of RGCs of wildtype animals after ONC but was not easily detected in sham retinas. To determine the efficiency of Jun recombination using Six3-cre, the number of JUN+ cells were counted 1 day after controlled optic nerve ONC, a time when JUN is widely expressed prior to RGC death. JUN+ cells are reduced by 77.7% in Jun deficient retinas after ONC (error bars represent standard error of the mean; n = 6 per genotype; P < 0.001; scale bar 50 μm). e/f Western blot analysis was used to determine the level of JUN in wildtype (+/+) and Jun deficient (−/−) animals. JUN was significantly reduced in both Jun deficient unmanipulated retinas (85%, ONC -) and Jun deficient retinas after ONC (92%, ONH +) as compared to wild type animals (* p < 0.01)

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