Fig. 3

Combined Jun/Ddit3 deficiency is more protective after axonal injury than either Jun or Ddit3 deficiency alone. a Example of TUJ1 staining in control and experimental eyes at 120 days after ONC (scale bar = 50 μm). b Ddit3, Jun, and combined Jun and Ddit3 deficient animals had significantly greater RGC survival than WT animals at all time points assessed (p < 0.001). Furthermore, there were significant differences (P < 0.001) found between the Ddit3, Jun, and combined Jun and Ddit3 groups at all time points evaluated expect for between the Jun, and combined Jun and Ddit3 14 days after ONC. Data are plotted as the percentage survival relative to sham animals (n = 8 per condition per genotype for 14, 35, and 60 days after ONC, except Jun deficient ONC n = 7 at 35 days after ONC and n ≥ 6 per condition per genotype for 120 days after ONC; error bars represent SEM). Raw data in RGCs per mm² and P values for all comparisons are presented in Table 1