Fig. 4

Increased activation of stress signaling molecules in TREM2 deficient hTau mice. a-d Western blot analysis was performed on microdissected cortices (a) and hippocampi (b) of hTau (Trem2 ^+/+) and hTau;Trem2 ^−/− mice using antibodies directed against phosphorylated and total JNK, ERK1/2, GSK3β, and P38 to determine relative levels of MAPK activation. Quantification of western blot data revealed highly significant increases in total levels of JNK, as well as the ratio of pJNK/total JNK, and also very highly significant increases in the ratio pGSK3β(Ser9)/GSK3β in cortex (c), while robust increases in total levels of GSK3β were accompanied by significant increases in the ratios pERK1/2/total ERK1/2, pGSK3β(Ser9)/Total GSK3β, pGSK3β(Y216)/total GSK3β, pP38/total P38, and pJNK/total JNK in hippocampi of 6-month mice (d). All experiments used n = 6 (equal males and females) mice per group unless otherwise noted. At least two independent experiments were performed for each analysis. Error bars represent SEM. ANOVA with Tukey correction *, P < 0.05, **, P < 0.01, ***, P < 0.001