Fig. 1

Synaptic and non-synaptic mitochondria are morphologically distinct in both rodent and human brain tissue. a and b. Example EM images of WT mouse and human cortical tissue displaying synaptic and non-synaptic mitochondria (S = synaptic, NS = non-synaptic). c and d. Scatterplots representing WT mouse (c.) and control human patient (d.) cortical synaptic and non-synaptic mitochondrial normalized areas (c. synaptic n = 391, non-synaptic n = 1113 from 4 mice; d. synaptic n = 177, non-synaptic n = 812 from 3 patients). A data point of distinct shape represents each individual animal or patient. Non-synaptic mitochondria appear to be significantly larger than synaptic mitochondria in both mouse and human cortical tissue. Statistical analyses used unpaired two-tailed Student’s t-tests with Welch correction for unequal sample size: ****p<0.0001, **p<0.01). Scale bars = 0.5 μm