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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Proteomic profiling of neuronal mitochondria reveals modulators of synaptic architecture

Fig. 2

Identification of the mitochondrial proteome. a. 2-way clustering of 1511 proteins identified in both synaptic (S) and non-synaptic (NS) mitochondrial samples. Each column represents a single LC-MS/MS run, based on log-transformed data. Proteins (rows) and samples (columns) are clustered by similarities in protein expression intensity, represented by the colour range: dark blue = least abundant, to dark red = most abundant. Dendrogram indicates protein expression differences between synaptic and non-synaptic mitochondrial sub-populations in addition to grouping of individual proteins that demonstrate similar intensity profiles. b. Graph represents expression of all 1511 identified proteins with 2 or more unique peptides, independent of magnitude. c. Graph displays the 411 proteins which have a > 2 fold change between populations, p < 0.05 and were identified with >1 unique peptide. The expression profile demonstrates 2 clear trends of differential protein expression between the sub-populations of mitochondria: proteins that are more abundant in synaptic mitochondria and less abundant in non-synaptic, and vice-versa. d and e. Principal components analyses of the unfiltered (d) and filtered (e) proteomic data. Pink data points denote non-synaptic mitochondrial samples and blue data points indicate synaptic mitochondrial samples. Samples are clustered by subcellular localization in both the filtered and unfiltered datasets with little technical variation. Grey displays identified proteins by accession number

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