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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Haplotype-specific MAPT exon 3 expression regulated by common intronic polymorphisms associated with Parkinsonian disorders

Fig. 3

Allele-specific RNA-protein complex formation by H1/H2 rs1800547 and rs17651213 sequences (a) RNA-protein complex formation by rs1800547 H1 (lane 2) and H2 (lane 12) biotin-labelled RNA probes with SK-N-F1 nuclear extract by RNA-EMSAs. The displacement of RNA-protein complex formation by unlabelled rs1800547 H1 (lanes 3–6, 17–20) or H2 (lanes 7–10, 13–16) competitor RNA probes of 10X, 20X, 40X and 100X in molar excess. The absence of SK-N-F1 nuclear extract as a negative control for RNA-protein complex formation (lanes 1 & 11). b RNA-protein complex formation by rs17651213 H1 (lane 2) and H2 (lane 8) biotin-labelled RNA probes with SK-N-F1 nuclear extract by RNA-EMSAs. The displacement of RNA-protein complex formation by unlabelled rs17651213 H1 (lanes 3, 4, 11, 12) or H2 (lanes 5, 6, 9, 10) competitor RNA probes of 500X and 1000X in molar excess. The absence of SK-N-F1 nuclear extract as a negative control for RNA-protein complex formation (lanes 1 & 7)

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Molecular Neurodegeneration

ISSN: 1750-1326