Fig. 2
From: Advances, challenges and future directions for stem cell therapy in amyotrophic lateral sclerosis

Isolation process to obtain ADSCs from human lipoaspirate. Fresh lipoaspirate is extensively washed in PBS to remove blood and contaminants. The adipose tissue is then enzymatically digested and the stromal vascular fraction (SVF) is obtained by filtration and centrifugation. Culture of the SVF in standard plastic tissue culture flasks results in the selection and expansion of the adipose stem cell population