Fig. 4
Soluble Aβ oligomer injection induces dendritic calcium transients with abnormally long duration and high peak amplitude during running. a. Schematic showing the injection of soluble Aβ oligomers in the brain and the imaging area contralateral to the inject site. b-c. Time-lapse images of dendritic calcium transients in vehicle (b) and Aβ injected mice (c) during running. Top, traces of calcium transient fluorescence during running. d-f. Distributions of the frequency (d), duration (e) and peak ΔF/F0 (f) of dendritic calcium transients between vehicle and Aβ oligomer-injected mice during treadmill running. Mice were infected with AAV to express GCaMP6s in layer 2/3 neurons in the motor cortex (Vehicle: 4 mice, 136 dendritic calcium transients; Aβ oligomer: 4 mice, 99 dendritic calcium transients. Mann-Whitney U Test). g-i. Distributions of the frequency (g), duration (h) and peak ΔF/F0 (i) of dendritic calcium transients in Aβ oligomer, Aβ monomer and vehicle-injected mice during treadmill running in Thy-1 GCaMP6 transgenic mice (Vehicle: 4 mice, 148 dendritic calcium transients; Aβ monomer: 4 mice, 176 dendritic calcium transients; Aβ oligomer: 4 mice, 152 dendritic calcium transients. Mann-Whitney U Test). ***P < 0.001. n.s., not significant