Fig. 12
From: A validated antibody panel for the characterization of tau post-translational modifications
Immunofluorescence staining reveals a large number of tau phosphorylation events between amino acids Y18 and S235 in hiPSC-derived neurons. All phospho-tau antibodies in the panel showed staining in hiPSC-derived neuronal cultures. While most antibodies stained both the cytoplasm and the cell body (a, c, d, e, f, g, h, i, j, k), pT181 (b) and pS235 (l) signals were exclusively in the neurites. Green: antibody staining, blue: Hoechst staining. Scale bars represent 100 μm