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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: A validated antibody panel for the characterization of tau post-translational modifications

Fig. 3

Peptide arrays validate the specificity of phospho-tau antibodies covering aa 181–231. a pT181, b pS198, c pS199, d pS202, e pS199/202, f pT205, g pT212, h pS214, i pT217 and j pT231 antibodies demonstrated specific detection of the respective modifications on a peptide array. While all antibodies are specific, the detection of pS198 (b), pS199 (c), pS202 (d) and pS214 (h) can be inhibited by additional phosphorylation events in the surrounding peptide sequence. The antibodies against pS202 (d), pS199/202 (e) and pT205 (f) also detect off-target modifications. The sequence and modification profile for peptides immobilized in each spot are shown below the blots. The modification targeted by the antibody is shown in red, off-target modifications are shown in bold black

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