Fig. 5

Peptide arrays demonstrate specificity issues for multiple phospho-tau antibodies. a Five different antibodies raised against pS262 detected the non-modified tau peptide A1. b The only available pT50 antibody detected non-modified peptides as well as those containing different modifications. c No signal was detected with the pY18 antibody for all peptides tested, suggesting that it may require a conformational epitope. d The only available pS400 antibody detected non-modified peptides A6 and A8. e All three different pS404 antibodies tested detected the non-modified peptides A1 and A7. f The only available antibody against pS422 detected the non-modified peptides A1 and A8 and also off-target modifications. Antibodies shown in (a, b, d, e and f) were discarded from the panel