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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: A validated antibody panel for the characterization of tau post-translational modifications

Fig. 6

Immunoblotting using hTg-Tau and Tau-KO brain lysates confirms the specificity for tau for a panel of antibodies. a) Scheme of tau (2N4R) showing the epitopes of the Tau12, Tau5, Tau1, HT7, BT2 and Dako antibodies. b) - d’) Immunoblots demonstrate strong reactivity of all antibodies with tau bands between 40 and 60 kD. Minor non-specific bands present in Tau-KO brain lysates were detected in d), e), i), h), p), x), y) and c’). Isoform-specific antibodies in h), i), j) and k) were validated using a tau ladder with all 6 recombinant human tau isoforms. kDa sizes for the marker are given in e’). Tg: hTg-Tau mouse, KO: Tau-KO mouse brain lysate, TL: recombinant tau ladder

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