Fig. 7
From: A validated antibody panel for the characterization of tau post-translational modifications
A number of antibodies failed to detect tau in hTg-Tau brain lysates. a Immunoblotting with recombinant tau (full length (1–441), truncated (1–421) and caspase 3 cleaved ((1–441) + Casp3) shows that all tau forms can be detected with a pan-tau antibody (Dako, left), whereas the C3-D421 antibody only detects the truncated and the caspase 3 cleaved form of tau (right). b and c Tau isoform-specific antibodies failed to detect tau in hTg-Tau brain lysates as well as the recombinant protein in the tau ladder. d, e, f and g No signal was detected by the meK311, pT212, pS238 and C3-D421 antibodies in hTg-Tau mouse brains, possibly due to the low abundance of these modifications. h Actin demonstrates equal loading for all samples. Tg: hTg-Tau mouse, KO: Tau-KO mouse brain lysate, TL: recombinant tau ladder