Fig. 9
From: A validated antibody panel for the characterization of tau post-translational modifications
An electrochemiluminescence ELISA detects tau PTMs in hTg-Tau mouse brain lysates. Sandwich ELISA assays using biotinylated antibodies for capture and sulfo-tagged Tau-12 antibody for detection demonstrate strong and specific signals for all antibodies in the panel. a Tau-12 cannot be used as capture and detection antibody at the same time, while all other total tau and tau isoform-specific antibodies in the panel are suitable to detect tau in hTg-Tau brain lysate. b All non-phospho-PTM modifications in the panel can be detected in hTg-Tau brain lysate. c All phospho-specific tau antibodies in the panel give signal from hTg-Tau brain lysate