A validated antibody panel for the characterization of tau post-translational modifications

Table 2 Localization of tau species in hiPSC-derived neurons

Name	Species	Company	Cat No.	Localization		Dilution
				Cell Body	Neurites
Tau12	mouse	Biolegend	SIG-39416	√	√	1:100
Tau5	mouse	Abcam	ab80579	n.s.	n.s.	1:50
Tau1	mouse	Millipore	MAB3420	√	√	1:100
Dako	rabbit	Agilent Dako	A0024	√	√	1:200
BT2	mouse	Thermo Fisher	MN1010	√	√	1:100
HT7	mouse	Thermo Fisher	MN1000	√	√	1:100
Tau-0 N	mouse	Biolegend	823,801	√	√	1:50
Tau-2 N	mouse	Biolegend	816,801	√	√	1:50
Tau-3R	mouse	Millipore	05–803	n.s.	n.s.	1:50
Tau-4R	mouse	Millipore	05–804	n.s.	n.s.	1:50
nY18	mouse	Biolegend	829,701	√	√	1:50
nY29	mouse	Millipore	MAB2244	√	√	1:50
acK280	rabbit	Anaspec	AS-56077	√		1:50
meK311	mouse	Biolegend	MMS-5102	√	√	1:50
C3-D421	mouse	Millipore	36–017	n.s.	n.s.	1:25
pY18	mouse	Novusbio	NBP2–42402	√	√	1:100
pT181	rabbit	Thermo Fisher	701,530		√	1:100
pS198	rabbit	Abcam	ab79540	√	√	1:100
pS199	rabbit	Thermo Fisher	701,054	√	√	1:100
pS202	rabbit	Anaspec	AS-28017	√	√	1:100
pS199/202	rabbit	Thermo Fisher	44-768G	√	√	1:100
pT205	rabbit	Abcam	ab181206	√	√	1:100
pT212	rabbit	Abcam	ab51053	√	√	1:100
pS214	rabbit	Thermo Fisher	PA5–35762	√	√	1:100
pT217	rabbit	Thermo Fisher	44–744	√	√	1:100
pT231	rabbit	Thermo Fisher	701,056	√	√	1:100
pS235	rabbit	Thermo Fisher	PIPA535761		√	1:100
pS238	mouse	Abcam	ab128889	√		1:100
pS356	rabbit	Abcam	ab51036	√	√	1:100
pS396	rabbit	Thermo Fisher	44-752G		√	1:100
pS400	rabbit	Anaspec	AS-54978		√	1:100
pS404	rabbit	Thermo Fisher	44-758G	√	√	1:100
pS409	rabbit	Abcam	ab4861	√	√	1:100
pS416	rabbit	Abcam	ab119391	√	√	1:100
pS422	rabbit	Abcam	ab79415		√	1:25

Summarizes the differential localization of tau species stained with pan-tau or PTM-specific tau antibodies in hiPSC-derived neurons (Figs. 10, 11, 12 and 13). n.s.: no signal

ISSN: 1750-1326