Fig. 10

Identification of molecular signaling pathways affected by Tau. At DIV30, hippocampal tissue extracts from OHSCs of control and anti-aggregant Tau^RDΔKPP slices, without DOX (switch-ON) and with DOX (switch-OFF) were subjected to western blot analysis of Wnt5a, Wnt3, and Tau (K9JA). Results show the mean ±SEM of 4-6 slices per animal and 6-8 animals per condition and represent the ratio between the analyzed protein and actin levels. Quantification was obtained by densitometry and analyzed by Student's t test. **p<0.01 and ***p<0.001. a There was a significant decrease (50%) in the level of Wnt5a in the anti-aggregant Tau^RDΔKPP slices compared to the controls (bar 1, 2). When the expression of anti-aggregant Tau^RD∆KPP was switched off by addition of DOX from DIV15 to DIV30, the levels of Wnt5a in the anti-aggregant Tau^RDΔKPP slices were further reduced by 30% (bar 3). b There was an increase (85%) in the Wnt3 levels in the anti-aggregant Tau^RDΔKPP slice cultures compared to control slices (bar 1, 2). There was a pronounced reduction (40% below the control levels) when the expression of anti-aggregant Tau^RD∆KPP was switched off from DIV15 to DIV30 (bar 3). c There was 80% increase in the Tau levels as detected by the pan Tau antibody K9JA in the anti-aggregant Tau^RDΔKPP slice cultures (bar 2). When the expression of anti-aggregant Tau^RDΔKPP was switched off from DIV15 to DIV30 the Tau level was reduced by 40% to that in the controls (bar 3)