Skip to main content
Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Anti-aggregant tau mutant promotes neurogenesis

Fig. 3

Decrease in microglial number in anti-aggregant TauRDΔKPP slices and increased microgliosis in the pro-aggregant TauRDΔK slices. OHSCs from control, anti-aggregant TauRDΔKPP and pro-aggregant TauRDΔK P8 pups were prepared and cultured until DIV30. Slices were stained with Iba1 antibody against microglia. a Representation of different types of microglia in the OHSCs. OHSCs from the control slices at DIV30 were immunostained with Iba1 (green) for microglia and NeuN (red) for neurons. The two panels on the left show the ramified form of microglia with multiple branches. The two right panels show the reactive form of microglia with fewer processes. Interestingly the last panel shows a microglia engulfing neuronal debris (note that the debris is stained red but appears yellow in the co-localization image). Scale bars = 10μm in all panels. b Overview of Iba1 staining of microglia (green) in the CA3 hippocampal region of control, anti-aggregant TauRDΔKPP and pro-aggregant TauRDΔK slices (scale bar 50μm). The controls and anti-aggregant TauRDΔKPP slices show ramified morphology of microglia whereas the pro-aggregant TauRDΔK slices had the reactive form of microglia. c Quantification of the number of Iba1 positive microglial cells in control, anti-aggregant TauRDΔKPP and pro-aggregant TauRDΔK slices at DIV30. All the Iba1 positive microglia were counted manually (as circled in the figure) by ImageJ. There was a reduction by 50% in the number of microglia in the anti-aggregant TauRDΔKPP slices at DIV30 compared to age-matched controls. By contrast, pro-aggregant TauRDΔK slices showed a massive increase of up to 100% in microglial number. Results are given as mean ±SEM of 10 animals and 4-6 slices per animal. Data were analyzed by Student's t-test., **p<0.01, ***p<0.001 and ****p<0.0001. d Quantification of the number of branches in microglia in control, anti-aggregant TauRDΔKPP and pro-aggregant TauRDΔK slices at DIV30. The branches were counted manually using the ImageJ software. The controls and the anti-aggregant TauRDΔKPP microglia showed 6-7 branches on average. By contrast, the pro-aggregant TauRDΔK microglia showed only 2-3 branches compared to the age-matched controls. Results are given as mean ±SEM of 10 animals and 4-6 slices per animal. Data were analyzed by Student's t-test ****p<0.0001 compared to control slices

Back to article page