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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Anti-aggregant tau mutant promotes neurogenesis

Fig. 4

Decrease of hypertrophic astrocytes in anti-aggregant TauRDΔKPP slices but no changes in the global GFAP content. a OHSCs from controls, anti-aggregant TauRDΔKPP and pro-aggregant TauRDΔK P8 pups were prepared and cultured until DIV30. Slices were stained with GFAP antibody (green) to label astrocytes. The control slices at DIV30 showed star-shaped astrocytes (left). In anti-aggregant TauRDΔKPP slices the astrocytes have smaller cell bodies and long, thin processes (middle). By contrast, pro-aggregant TauRDΔK slices showed hypertrophic astrocytes (right) with fewer processes which are typical of neurodegeneration. b Biochemical analysis of GFAP protein levels. Protein extracts from OHSCs of control, anti-aggregant TauRDΔKPP and pro-aggregant TauRDΔK slices at DIV30 were subjected to western blot (loading 3μg/lane) to analyze for GFAP protein levels. c Quantification of the western blots showing that the GFAP protein contents of the three samples are similar. Results are given as mean ±SEM of 4-6 slices per mice and 5-6 mice and represent the ratio between GFAP and actin levels. Data were analyzed by Student's t-test

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