Fig. 8
Proliferation of BrdU positive cells in P8 animals. P8 animals were given intra-peritoneal injections (i.p) of BrdU (50mg/kg body weight) 2 hours before sacrifice. The whole brain was cut into 30μM thick vibratome sections and slices were stained for neurons (NeuN), Tau (K9JA) and BrdU (BrdU antibody). a Representative images of NeuN (green), K9JA (red) and BrdU (cyan) staining in the CA1, CA3 and DG areas of the hippocampus of P8 control and anti-aggregant TauRD∆KPP mice. Scale bar 50μm. b Number of BrdU positive cells was counted by stereology. An increase (80%) was observed in the CA3 region in the anti-aggregant TauRDΔKPP pups compared to controls (compare bars 3, 4). Data were analyzed by Student's t test. *p<0.05 compared to controls. c Volume of the hippocampus in control and anti-aggregant TauRDΔKPP P8 pups. An increase in the volume of the hippocampus (25%) was observed in anti-aggregant TauRDΔKPP animals. The values determined are the apparent volumes of hippocampus. Results show the mean ±SEM (n= 3-5 animals/group) and data were analyzed by Student's t test. *p<0.05 compared to controls