Fig. 5
Effect of GBA overexpression on susceptibility of GBA+/L444P mice to MPTP-induced PD-like symptoms. a Representative photomicrographs from coronal mesencephalon sections containing TH-positive neurons in AAV-Con injected WT, AAV-GBA injected WT, AAV-Con injected GBA+/L444P, and AAV-GBA injected GBA+/L444P mice treated with saline or MPTP, respectively (scale bar, 500 μm). b Stereology counts of TH, and c Nissl-positive neurons in the SNpc region. Unbiased stereologic counting was performed in the SNpc region. d Representative photomicrograph of striatal sections stained for TH immunoreactivity with low (scale bar, 100 μm) and high magnification (scale bar, 50 μm). e Quantification of dopaminergic fiber densities in the striatum using Image J software (NIH). a-e Error bars represent means ± S.E.M (n = five mice per group). f Pole test was conducted on the sixth day post MPTP injection. g Grip strength test was conducted on the sixth day post MPTP injection. Behavioral abnormalities and susceptibility in pole test and grip strength test induced by MPTP injection were ameliorated in WT and GBA+/L444P mice with AAV-GBA. f, g Error bars represent the mean ± S.E.M (six or seven mice per group for behavioral studies). Two-way ANOVA was used for statistical analysis followed by post-hoc Bonferroni test for multiple group comparison. *P < 0.05, **P < 0.01, ***P < 0.001 vs. MPTP-treated WT group with AAV-Con or MPTP-treated GBA+/L444P group with AAV-Con. n.s: not significant