Fig. 1
From: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Pathogenic LRRK2 causes deficits in differentiation and altered centrosome positioning in differentiated SH-SY5Y cells. a Quantification of neurite length in SH-SY5Y cells differentiated with retinoic acid for 5 days. Around 150 cells were analyzed per condition and experiment. Bars represent mean ± s.e.m., (n = 3 independent experiments); *, p < 0.05. b Quantification of neurite length only including differentiated cells. Around 150 differentiated cells were analyzed per condition and experiment. Bars represent mean ± s.e.m., (n = 3 independent experiments). c Quantification of percentage of differentiated cells. Bars represent mean ± s.e.m., (n = 3 independent experiments); *, p < 0.05. d Quantification of differentiated cells where centrosome is positioned on the side or opposite the longest neurite in the distinct cells as indicated. Around 30 non-contiguous differentiated cells were analyzed per condition and experiment. Bars represent mean ± s.e.m., (n = 3 independent experiments); *, p < 0.05. e Example of non-contiguous SH-SY5Y cells stably expressing GFP, or flag-tagged wildtype or G2019S-mutant LRRK2 as indicated, and stained for pericentrin and DAPI. Scale bar, 10 μm