Fig. 1
From: Piperine ameliorates SCA17 neuropathology by reducing ER stress
MANF overexpression extended lifespan and ameliorated ER stress in TBP KI mice. a Kaplan-Meier survival curve shows the lifespan of TBP KI and TBP KI/MANF mice (n = 10 for each phenotype, including 5 males and 5 females). b Mean body weight of 4-month-old WT, TBP KI and TBP KI/MANF mice (n = 10 for each phenotype, including 5 males and 5 females). * P < 0.05, ** P < 0.01. c Western blotting analysis of the expression of N-terminal ATF6 fragment (ATF6(N)) in the cortex and cerebellum of 4-month-old WT and TBP KI mice. Actin was used as a loading control. d Quantification of the ratio of ATF6(N) to actin on western blots in Fig. 1c (n = 3, * P < 0.05). e Western blotting analysis of the expression of ATF6(N) in the cortex and cerebellum of 4-month-old TBP KI and TBP KI/MANF mice. f Quantification of the ratio of ATF6(N) to actin on western blots in Fig. 1e (n = 3, * P < 0.05). g Immunohistochemistry staining of ATF6 in the cortex of 4-month-old WT, TBP KI and TBP KI/MANF mice (Scale bar: 20 μm)